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QIAGEN EndoFree Plasmid Mega Kit無內(nèi)毒素質(zhì)粒提取試劑盒

  • 更新時間:  2023-07-26
  • 產(chǎn)品型號:  12381
  • 簡單描述
  • QIAGEN EndoFree Plasmid Mega Kit無內(nèi)毒素質(zhì)粒提取試劑盒
    EndoFree Plasmid Kits基于陰離子交換技術(shù),可快速制備不含內(nèi)毒素的質(zhì)粒DNA。QIAfilter Cartridges過濾快速澄清裂解液。制備的DNA純度超過兩次CsCl梯度離心法所得DNA的純度,適合超轉(zhuǎn)染級純的應(yīng)用。EndoFree Plasmid Buffer Set可用于制備10次m
詳細介紹

QIAGEN EndoFree Plasmid Mega Kit無內(nèi)毒素質(zhì)粒提取試劑盒

純化得到至多10 mg的無內(nèi)毒素高轉(zhuǎn)染級純質(zhì)?;蚩滤官|(zhì)粒DNA

  • 純化得到的DNA內(nèi)毒素水平低于0.1 EU/µg
  • 純化流程快,同時去除內(nèi)毒素
  • 獲得高產(chǎn)量、高拷貝質(zhì)粒DNA
  • 使用LyseBlue,獲得的裂解和高產(chǎn)量的DNA

QIAGEN EndoFree Plasmid Mega Kit無內(nèi)毒素質(zhì)粒提取試劑盒

質(zhì)粒純化方法與轉(zhuǎn)染效率。

使用兩種方法獨立制備pRSVcat,分別使用脂質(zhì)體介導的方法轉(zhuǎn)染到COS-7、HeLa和LMH細胞中,及使用磷酸鈣轉(zhuǎn)染至Huh7細胞中。平均轉(zhuǎn)染效率以相對于QIAGEN Plasmid Kit(100%)制備的DNA的轉(zhuǎn)染效率表示。

質(zhì)粒純化方法與轉(zhuǎn)染效率。

使用兩種方法獨立制備pRSVcat,分別使用脂質(zhì)體介導的方法轉(zhuǎn)染到COS-7、HeLa和LMH細胞中,及使用磷酸鈣轉(zhuǎn)染至Huh7細胞中。平均轉(zhuǎn)染效率以相對于QIAGEN Plasmid Kit(100%)制備的DNA的轉(zhuǎn)染效率表示。

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